2024-12-03
Flowchart of data aquisition and inital tidying
Flowchart of data augmentation
(A) Overall cellular composition of the MDS patient samples as well as healthy donors
(B) The plots depicts the total number as well as the fraction of MDS and HD samples that contain the srsf2 and sf3b1 mutations within the various cellular compartments.
The histograms above illustrate gene counts from both MDS patients and healthy donors, displayed before and after normalization.
The impact of normalization appears subtle. However, it is noticeable that the overall gene counts in MDS patients are comparatively higher than those in healthy donors.
Several genes are differentially expressed between healthy donors and patients with MDS in the four analyzed cell populations.
Single cell analysis (RNASeq or proteomics) would provide further detail.
Differences in expression between the SF3B1/SRSF2 mutants and wild types?
More sample/clinical data on MDS samples would allow to analyze these more in deeply and gain more knowledge of differential gene expression between different classes of MDS.
22160 - R for Bio Data Science